Functional scFv antibody sequences against the organophosphorus pesticide chlorpyrifos

被引：9

作者：

Alcocer, MJC ^{[1
]}

Doyen, C ^{[1
]}

Lee, HA ^{[1
]}

Morgan, MRA ^{[1
]}

机构：

[1] Inst Food Res, Norwich NR4 7UA, Norfolk, England

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2000年 / 48卷 / 02期

关键词：

variable region; antibody engineering; immunoassay; recombinant antibody; antibody pseudogene;

D O I：

10.1021/jf990690u

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Two functional single-chain Fv (scFv) antibodies that recognize specifically the widely used organophosphorus pesticide chlorpyrifos-ethyl were derived from two murine hybridoma cell lines. It is shown that the functional scFvs could be isolated without any rounds of selection, with a success rate dependent on the efficiency of amplification of the functional light chain gene family by the specific primers. Besides four new functional immunoglobulin variable gene sequences, the isolation of a new pseudogene is reported.

引用

收藏

页码：335 / 337

页数：3

相关论文

共 16 条

[1] HYBRIDOMA FUSION CELL-LINES CONTAIN AN ABERRANT KAPPA TRANSCRIPT [J].

CARROLL, WL ;

MENDEL, E ;

LEVY, S .

MOLECULAR IMMUNOLOGY, 1988, 25 (10) :991-995

[2] A NOVEL STRATEGY FOR THE IMMUNOLOGICAL TAGGING OF CDNA CONSTRUCTS [J].

CRAVCHIK, A ;

MATUS, A .

GENE, 1993, 137 (01) :139-143

[3] ELIMINATION OF ENDOGENOUS ABERRANT KAPPA-CHAIN TRANSCRIPTS FROM SP2/0-DERIVED HYBRIDOMA CELLS BY SPECIFIC RIBOZYME CLEAVAGE - UTILITY IN GENETIC THERAPY OF HIV-1 INFECTIONS [J].

DUAN, LX ;

POMERANTZ, RJ .

NUCLEIC ACIDS RESEARCH, 1994, 22 (24) :5433-5438

[4] Production of a recombinant anti-parathion antibody (scFv); Stability in methanolic food extracts and comparison to an anti-parathion monoclonal antibody [J].

Garrett, SD ;

Appleford, DJA ;

Wyatt, GM ;

Lee, HA ;

Morgan, MRA .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1997, 45 (10) :4183-4189

[5]

Harrison JL, 1996, METHOD ENZYMOL, V267, P83

[6] DEVELOPMENT AND APPLICATION OF LABORATORY AND FIELD IMMUNOASSAYS FOR CHLORPYRIFOS IN WATER AND SOIL MATRICES [J].

HILL, AS ;

SKERRITT, JH ;

BUSHWAY, RJ ;

PASK, W ;

LARKIN, KA ;

THOMAS, M ;

KORTH, W ;

BOWMER, K .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1994, 42 (09) :2051-2058

[7] Recombinant antibody fragments [J].

Hudson, PJ .

CURRENT OPINION IN BIOTECHNOLOGY, 1998, 9 (04) :395-402

[8]

Huston J S, 1993, Int Rev Immunol, V10, P195, DOI 10.3109/08830189309061696

[9]

Kabat E. A., 1991, SEQUENCES PROTEINS I

[10] Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system [J].

Krebber, A ;

Bornhauser, S ;

Burmester, J ;

Honegger, A ;

Willuda, J ;

Bosshard, HR ;

Pluckthun, A .

JOURNAL OF IMMUNOLOGICAL METHODS, 1997, 201 (01) :35-55