Effect of mammalian cell culture medium on the gelation properties of Pluronic® F127

被引：101

作者：

Matthew, JE ^{[1
]}

Nazario, YL ^{[1
]}

Roberts, SC ^{[1
]}

Bhatia, SR ^{[1
]}

机构：

[1] Univ Massachusetts, Dept Chem Engn, Amherst, MA 01003 USA

来源：

BIOMATERIALS | 2002年 / 23卷 / 23期

关键词：

sol-gel boundary; cell culture; encapsulation; PEO-PPO-PEO; triblock copolymers;

D O I：

10.1016/S0142-9612(02)00208-9

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We investigate the gelation of a poly(ethylene oxide)-polypropylene oxide)-polyethylene oxide) copolymer, Pluronic(R) F127, in mammalian cell culture medium for applications in tissue engineering and cell encapsulation. In both minimum essential medium (MEM) and MEM with added fetal bovine serum (MEM-FBS), the gel-phase boundary shifts to lower temperatures and concentrations as compared to pure water. The thermodynamics of gel formation are similar in MEM, MEM-FBS, and pure water, suggesting that the mechanism of gelation is similar in all three solvents. The shift of the sol-gel boundary to lower concentrations is particularly significant for development of cell encapsulation protocols using Pluronics(R) and applications where copolymer concentration must be minimized due to toxicity concerns. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：4615 / 4619

页数：5

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