Raman spectroscopic signature of life in a living yeast cell

被引：92

作者：

Huang, YS

Karashima, T

Yamamoto, M

Ogura, T

Hamaguchi, H

机构：

[1] Univ Tokyo, Dept Chem, Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Dept Biophys & Biochem, Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

[3] Univ Hyogo, Grad Sch Sci, Dept Life Sci, Kamigori, Hyogo 6781297, Japan

来源：

JOURNAL OF RAMAN SPECTROSCOPY | 2004年 / 35卷 / 07期

关键词：

time- and space-resolved Raman spectroscopy; single living cell; cell cycle; fission yeast; cellular respiration inhibition;

D O I：

10.1002/jrs.1219

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

We have discovered a Raman spectroscopic signature that sharply reflects the metabolic activity of a mitochondrion in a living yeast cell. Raman mapping experiments on a GFP-labeled yeast cell showed that this signature originated exclusively from mitochondria. Addition of KCN caused a rapid decrease and subsequent disappearance of the signature followed by gradual changes of the phospholipid Raman bands, indicating that respiration was first inhibited by KCN and then lowered metabolic activity gradually deteriorated the double-membrane structure of a mitochondrion. We can now monitor the life and death of a single cell by time- and space-resolved Raman spectroscopy. Copyright (C) 2004 John Wiley Sons, Ltd.

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页码：525 / +

页数：3

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