The alkaloid matrine of the root of Sophora flavescens prevents arrhythmogenic effect of ouabain

被引：76

作者：

Zhou, Yuhong ^{[1
]}

Wu, Yun ^{[2
]}

Deng, Lin ^{[1
]}

Chen, Lanlan ^{[1
]}

Zhao, Dandan ^{[1
]}

Lv, Lifang ^{[1
]}

Chen, Xu ^{[1
]}

Man, Jinyu ^{[3
]}

Wang, Yansong ^{[3
]}

Shan, Hongli ^{[1
]}

Lu, Yanjie ^{[1
]}

机构：

[1] Harbin Med Univ, Minist Educ, Key Lab Cardiovasc Res,Dept Pharmacol, State Prov Key Labs Biomed Pharmaceut China, Harbin 150081, Heilongjiang, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 2, Dept Internal Neurol, Harbin 150081, Heilongjiang, Peoples R China

[3] Harbin Med Univ, Affiliated Hosp 1, Dept Anaesthesia, Harbin 150081, Heilongjiang, Peoples R China

来源：

PHYTOMEDICINE | 2014年 / 21卷 / 07期

基金：

中国国家自然科学基金;

关键词：

Matrine; Alkaloid; Sophora flavescens; Ouabain; L-type Ca2+ current; Ca2+ transients; ARRHYTHMIAS; MECHANISMS; MYOCYTES; EFFICACY; MICE; AIT;

D O I：

10.1016/j.phymed.2014.02.008

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Matrine, a alkaloid of the root of Sophora flavescens, has multiple protective effects on the cardiovascular system including cardiac arrhythmias. However, the molecular and ionic mechanisms of matrine have not been well investigated. Our study aimed at to shed a light on the issue to investigate the antiarrhythmic effects of matrine by using ouabain to construct an arrhythmic model of cardiomyocytes. In this experiment, matrine significantly and dose-dependently increased the doses of ouabain required to induce cardiac arrhythmias and decreased the duration of arrhythmias in guinea pigs. In cardiomyocytes of guinea pigs, ouabain 10 mu M prolonged action potential duration by 80% (p < 0.05) and increased L-type Ca2+ currents and Ca2+ transients induced by KCl (p < 0.05). Matrine 100 mu M shortened the prolongation of APD and prevented the increase of L-type Ca2+ currents and Ca2+ transients induced by ouabain. Taken together, these findings provide the first evidence that matrine possessed arrhythmogenic effect of ouabain by inhibiting of L-type Ca2+ currents and Ca2+ overload in guinea pigs. (C) 2014 Elsevier GmbH. All rights reserved.

引用

页码：931 / 935

页数：5

共 23 条

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Transgenic simulation of human heart failure-like L-type Ca2+-channels: implications for fibrosis and heart rate in mice [J].