Comparison of antibody and aptamer receptors for the specific detection of thrombin with a nanometer gap-sized impedance biosensor

被引：62

作者：

Schlecht, U. ^{[1
]}

Malave, A. ^{[1
]}

Gronewold, T. ^{[1
]}

Tewes, M. ^{[1
]}

Loehndorf, M. ^{[1
]}

机构：

[1] CAESAR, D-53175 Bonn, Germany

来源：

ANALYTICA CHIMICA ACTA | 2006年 / 573卷

关键词：

impedance; biosensor; nanogap; thrombin; protein-interaction;

D O I：

10.1016/j.aca.2006.01.016

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Nanogap-impedance biosensors with electrode separations of 75 nm have been fabricated by means of standard optical lithography and a sacrificial layer technique. Due to a large surface-to-volume ratio and high sensitivity, these sensors are superior compared to open interdigitated electrode structures. As a model, the blood coagulation factor thrombin was detected. As specific receptors, either an antibody or a RNA-aptamer have been used. The microwave frequency impedance measurements showed that both ligands were equally suitable for the specific detection of thrombin. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：65 / 68

页数：4

共 16 条

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