Genomic and proteomic analyses reveal multiple homologs of genes encoding enzymes of the methanol:coenzyme M methyltransferase system that are differentially expressed in methanol- and acetate-grown Methanosarcina thermophila

被引：27

作者：

Ding, YHR

Zhang, SP

Tomb, JF

Ferry, JG ^{[1
]}

机构：

[1] Penn State Univ, Dept Biochem & Mol Biol, Eberly Coll Sci, S Frear Lab 205, University Pk, PA 16802 USA

[2] DuPont Co Inc, Cent Res & Dev, Expt Stn, Wilmington, DE 19880 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 215卷 / 01期

基金：

美国国家科学基金会;

关键词：

archaea; Methanogenesis; acetate; methanol; trimethylamine; two-dimensional gel electrophoresis;

D O I：

10.1016/S0378-1097(02)00924-2

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Each of the genomic sequences of Methanosarcina acetivorans, Methanosareina mazei, and Methanosareina thermophila revealed two homologs of mtaA, three homologs of mtaB, and three homologs of mtaC encoding enzymes specific for methanogenesis from methanol. Two-dimensional gel electrophoretic analyses of polypeptides from M. thermophila established that methanol induces the expression of mtaA-1, mtaB-1, mtaB-2, mtaB-3, mtaC-1, mtaC-2, and mtaC-3 whereas mtaB-3 and mtaC-3 are constitutively expressed in acetate-grown cells. The gene product of one of three mttC homologs, encoding trimethylamine-specific methyltransferase I, was detected in methanol-but not acetate-grown M. thermophila. A postulated role for the multiple homologs is discussed. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：127 / 132

页数：6

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