Quantitative PCR (qPCR) and the Guide to good practices MIQE: adapting and relevance in the clinical biology context

被引：17

作者：

Dooms, Maxime ^{[1
]}

Chango, Abalo ^{[1
]}

Abdel-Nour, Afif ^{[2
]}

机构：

[1] Inst Polytech LaSalle Beauvais, Dept SNES, Unite Rech EGEAL UP 2012 10 101, Beauvais, France

[2] King Abdulaziz Univ, King Fahd Med Res Ctr, Special Infect Dis Unit, Jeddah 21413, Saudi Arabia

来源：

ANNALES DE BIOLOGIE CLINIQUE | 2014年 / 72卷 / 03期

关键词：

best practice; guideline; MIQE; PCR; quantitative PCR; MINIMUM INFORMATION; PUBLICATION;

D O I：

10.1684/abc.2014.0955

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The qPCR has been introduced in clinical and biomedical research for over 10 years from now. Its use in trials and diagnostics is continuously increasing. Due to this heavy use, the question of relyability and relevance of qPCR results has to be asked. This review proposes a documented and evidence based answer to this question, thanks to the MIQE (minimum information for publication of quantitative real-time PCR experiments) guideline. The whole analysis process is addressed, from nucleic acids extraction to data management. Simple answers are given, taking into account the technical constraints from clinical research in order to allow a realistic application of this guideline.

引用

页码：265 / 269

页数：5

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