In-Gel 18O Labeling for Improved Identification of Proteins from 2-DE Gel Spots in Comparative Proteomic Experiments

被引：6

作者：

Broedel, Oliver ^{[1
,2
]}

Krause, Eberhard ^{[3
]}

Stephanowitz, Heike ^{[3
]}

Schuemann, Michael ^{[3
]}

Eravci, Murat ^{[1
,2
]}

Weist, Stephanie ^{[1
,2
]}

Brunkau, Cindy ^{[2
]}

Wittke, Janosch ^{[2
]}

Eravci, Selda ^{[2
]}

Baumgartner, Andreas ^{[1
,2
]}

机构：

[1] Charite Univ Med Berlin, Dept Radiol & Nucl Med Radiochem, D-12200 Berlin, Germany

[2] AM Proteome Sci, Berlin, Germany

[3] Leibniz Inst Mol Pharmacol FMP, Berlin, Germany

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 07期

关键词：

O-18; comparative proteomics; mass spectrometry; quantification; two-dimensional gel electrophoresis; TANDEM MASS-SPECTROMETRY; RELATIVE QUANTIFICATION; QUANTITATIVE PROTEOMICS; MITOCHONDRIAL PROTEINS; ELECTROPHORESIS; STRATEGIES;

D O I：

10.1021/pr8010765

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The reliability of 2-DE gel-based comparative proteornics is severely impaired by the potential presence of overlapping proteins. We describe a methodological procedure which may solve this problem. Corresponding protein spots from two experimental groups are digested in the presence of O-16 and O-18, respectively. Samples are pooled and proteins identified by MS. The O-18/O-16-ratios of the different proteins found in the same spot distinguish proteins with altered from those whose intensity is unchanged.

引用

页码：3771 / 3777

页数：7

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