Cloning and sequencing of the serine dehydrogenase gene from Agrobacterium tumefaciens

被引：5

作者：

Fujisawa, H ^{[1
]}

Nagata, S ^{[1
]}

Chowdhury, EK ^{[1
]}

Matsumoto, M ^{[1
]}

Misono, H ^{[1
]}

机构：

[1] Kochi Univ, Dept Bioresource Sci, Nankoku, Kochi 7838502, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2002年 / 66卷 / 05期

关键词：

serine dehydrogenase; Agrobacterium tumefaciens; short-chain alcohol dehydrogenase; nucleotide sequence; amino acid sequence;

D O I：

10.1271/bbb.66.1137

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structural gene for NADP(+)-dependent serine dehydrogenase [EC 1.1.1.-] from Agrobacterium tumefaciens ICR 1600 was cloned into Escherichia coli cells and its complete DNA sequence was analyzed. The gene encodes a polypeptide containing 249 amino acid residues. The enzyme had high sequence similarity to short-chain alcohol dehydrogenases from bacteria and unknown proteins of Haemophilus influenzae, Escherichia coli, and Saccharomyces cerevisiae.

引用

页码：1137 / 1139

页数：3