Integrity and activity of photosystem 2 complexes isolated from the thermophilic cyanobacterium Synechococcus elongatus using various detergents

被引:13
作者
Setlíková, E [1 ]
Sofrová, D
Prásil, O
Budác, P
Koblízek, M
Setlík, I
机构
[1] Acad Sci Czech Republ, Inst Microbiol, Dept Autotroph Microorganisms, CZ-37981 Trebon, Czech Republic
[2] Charles Univ, Fac Sci, Dept Biochem, CZ-12840 Prague, Czech Republic
[3] Univ S Bohemia, Fac Biol, CZ-37005 Ceske Budejovice, Czech Republic
关键词
fluorescence induction; low temperature fluorescence spectra; oxygen evolution; PQ pool; Q(A)(-) reoxidation; Q(B) pocket; thermoluminescence;
D O I
10.1023/A:1007047703388
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The efficiency in selective extraction of photosystem (PS) 2 oxygen evolving complexes was compared among seven detergents. These were applied to thylakoid membranes of the thermophilic cyanobacterium Synechococcus elongatus, Used were five non-ionic detergents with one ionic and one zwitterionic for comparison. To compare the suitability and efficiency of the detergents the following properties of the extracts were examined: maximum rate of oxygen evolution with various electron accepters, the relative variable fluorescence (F-V/F-M), the contamination of the extract with photosystem (PS) 1, and the status of the electron acceptor side of PS2 reaction centre, None of the detergents yielded a highly selective extraction of the PS2 complexes (negligible contamination with PS1) which would simultaneously display a high photochemical activity and high structural intactness. Heptylthioglucoside and dodecylmaltoside yielded the nearest approximation to the optimum result. Kinetic fluorometry was applied here for the first time to characterize the functional and structural properties of PS2 particles from cyanobacteria.
引用
收藏
页码:183 / 200
页数:18
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