Monitoring molecular recognition of the ribosomal decoding site

被引：120

作者：

Shandrick, S

Zhao, Q

Han, Q

Ayida, BK

Takahashi, M

Winters, GC

Simonsen, KB

Vourloumis, D

Hermann, T

机构：

[1] Anadys Pharmaceut Inc, Dept Struct Chem, San Diego, CA 92121 USA

[2] Anadys Pharmaceut Inc, Dept Med Chem, San Diego, CA 92121 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 24期

关键词：

antibiotics; fluorescent probes; molecular recognition; RNA structures; structure elucidation;

D O I：

10.1002/anie.200454217

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Adenine flips out: A combination of X-ray crystallography, 2-aminopurine fluorescence labeling, and the use of aminoglycosides as ligands is exploited to demonstrate conformational transitions in the RNA domain that ensures accurate protein synthesis (see picture). The triggering of a conformational change of an adenine unit in the RNA by ligand binding can be used as the basis of a screening method to discover antibiotics.

引用

收藏

页码：3177 / 3182

页数：6

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