In Vitro Studies on Regulation of Osteogenic Activities by Electrical Stimulus on Biodegradable Electroactive Polyelectrolyte Multilayers

被引：80

作者：

Cui, Haitao ^{[1
,2
]}

Wang, Yu ^{[1
]}

Cui, Liguo ^{[1
]}

Zhang, Peibiao ^{[1
]}

Wang, Xianhong ^{[1
]}

Wei, Yen ^{[3
]}

Chen, Xuesi ^{[1
]}

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100039, Peoples R China

[3] Tsinghua Univ, Dept Chem, Beijing 100084, Peoples R China

来源：

BIOMACROMOLECULES | 2014年 / 15卷 / 08期

基金：

中国国家自然科学基金;

关键词：

PERCUTANEOUS INTRAMUSCULAR ELECTRODES; MESENCHYMAL STROMAL CELLS; TISSUE REGENERATION; MYOBLAST DIFFERENTIATION; BIOMEDICAL APPLICATIONS; EXPONENTIAL-GROWTH; CONDUCTING POLYMER; CARBON NANOTUBES; HUMAN MSCS; STIMULATION;

D O I：

10.1021/bm5007695

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

In this study, a novel electroactive tetreaniline-containing degradable polyelectrolyte multilayer film (PEM) coating [(poly(L-glutamic acid)-graft-tetreaniline/poly(L-lysine)-graft-tetreaniline)(n), (PGA-g-TA/PLL-g-TA)(n)] was designed and fabricated by layer-by-layer (LbL) assembly method. Compared with the nongrafted PEMs, the tetreaniline-grafted PEMs showed higher roughness and stiffness in micro/nanoscale structures. The special surface characteristics and the typical electroconductive properties were more beneficial for adhesion, proliferation, and differentiation of preosteoblast MC3T3-E1 cells. Moreover, the enhanced effects were observed on the modulation of MC3T3-E1 cells that differentiated into maturing osteoblasts, when the electroactive PEMs were coupled with electrical stimulus (ES), especially in the early phase of the osteoblast differentiation. The alkaline phosphatase (ALP) activity, calcium deposition, immunofluorescence staining, and RT-qPCR were evaluated on the differentiation of preosteoblast. These data indicate that the comprehensive effects through coupling electroactive scaffolds with electrical stimulus are better to develop bioelectric strategies to control cell functions for bone regeneration.

引用

页码：3146 / 3157

页数：12

共 54 条

[31]

Increased Proliferation and Differentiation of Pre-Osteoblasts MC3T3-E1 Cells on Nanostructured Polypyrrole Membrane Under Combined Electrical and Mechanical Stimulation [J].