Interleukin-1 beta-converting enzyme and CPP32 are involved in ultraviolet B-induced apoptosis of SV40-transformed human keratinocytes

Interleukin-1 beta-converting enzyme (ICE) and CPP32 are cysteine proteinases, that are involved in apoptotic process in various cell systems, We investigated the effects of ICE on ultraviolet B (UVB) induced-apoptosis in SV40-transformed human keratinocytes (SVHK cells). The ICE inhibitor (Z-Val-Ala-Asp-CH2F) and CPP32 inhibitor (Z-Asp-Glu-Val-Asp-CH2F) blocked the apoptotic cell death caused by UVB irradiation, The addition of both ICE and CPP32 inhibitors to the incubation medium resulted in neither an additive nor a synergistic suppression of UVB-induced apoptosis. Reverse transcription and polymerase chain reaction (RT-PCR) analysis indicated that SVHK cells ex-pressed ICE-alpha, and beta mRNAs, UVB irradiation increased the mRNA of both isoforms and Western blot analysis confirmed that UVB increased an active form of ICE protein, p20, that is generated by autoproteolytic cleavage of inactive 45 kDa proenzyme derived from both mRNAs, Transfection of ICE expression vector into SVHK cells resulted in apoptosis in a dose depended manner and UVB-irradiation further augmented the ICE expression vector-induced apoptosis. These results indicate that ICE plays an important role in UVB-induced apoptosis of SVHK cells. (C) 1997 Academic Press.