Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter

被引:17
作者
Yang, SH [1 ]
Yu, H [1 ]
Goh, CJ [1 ]
机构
[1] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Plant Growth & Dev Lab, Singapore 119260, Singapore
关键词
cytokinin oxidase; orchid; regulation;
D O I
10.1093/jxb/erf055
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The orchid DSCKX1 is a new member of the cytokinin oxidase gene family, which catalyses the degradation of cytokinins bearing unsaturated isoprenoid side chains. A 3.7 kb fragment upstream of the DSCKX1 coding region was isolated, sequenced and characterized by deletion analysis of DSCKX1::beta-glucuronidase gene fusions using transient orchid and stable Arabidopsis transformation systems. Functional analysis of 5' deletions defined the 5'-upstream region that directs the expression in distinct tissues. Regulatory elements affecting the cytokinin induction of the DSCKX1 gene have also been delineated.
引用
收藏
页码:1899 / 1907
页数:9
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