Separation of nontarget compounds by DNA aptamers

被引：75

作者：

Kotia, RB ^{[1
]}

Li, LJ ^{[1
]}

McGown, LB ^{[1
]}

机构：

[1] Duke Univ, Dept Chem, Paul M Gross Chem Lab, Durham, NC 27708 USA

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 04期

关键词：

D O I：

10.1021/ac991112f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The ability of DNA aptamers to separate nontarget compounds is demonstrated. Two G-quartet forming aptamers, a 15-mer and a 20-mer, were covalently linked to fused silica capillary columns to serve as stationary-phase reagents in capillary electrochromatography. Separations of binary mixtures of amino acids (D-trp and D-tyr), enantiomers (D-trp and L-trp), and polycyclic aromatic hydrocarbons were achieved. Aptamers offer several attractive features for stationary-phase reagents, including ease of synthesis and of attachment to surfaces and modification of their binding properties through minor changes in sequence.

引用

页码：827 / 831

页数：5

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