Regulation of Na+ channel beta 1 and beta 2 subunit mRNA levels in cultured rat astrocytes

被引：19

作者：

Oh, Y

Lee, YJ

Waxman, SG

机构：

[1] UNIV ALABAMA,NEUROBIOL RES CTR,BIRMINGHAM,AL 35294

[2] YALE UNIV,SCH MED,DEPT NEUROL,NEW HAVEN,CT 06510

[3] VA HOSP,NEUROSCI RES CTR,W HAVEN,CT 06516

来源：

NEUROSCIENCE LETTERS | 1997年 / 234卷 / 2-3期

关键词：

ion channel; spinal cord; quantitative reverse transcription-polymerase chain reaction; cyclic adenosine monophosphate; calcium ionophore;

D O I：

10.1016/S0304-3940(97)00694-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Using quantitative competitive reverse transcription-polymerase chain reaction (RT-PCR), we found an increased level of Na+ channel beta 1 (Na beta 1) mRNAs in spinal cord astrocytes and in the B50 neuroblastoma cell line after exposure to 1 mM dibutyryl cAMP. In contrast, the calcium ionophore (1 mu M A23187) did not affect Na beta 1 mRNA levels in these cells. Further, we amplified full length coding region of Na+ channel beta 2 (Na beta 2) mRNA from rat optic nerve and cultured astrocytes using RT-PCR. It appeared that the Na beta 2 mRNA level was increased by dibutyryl cAMP, but not by A23187, in spinal cord astrocytes. These findings suggest that the Na beta 1 and Na beta 2 mRNA levels in spinal cord astrocytes are influenced by increased cAMP but not by calcium. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：107 / 110

页数：4

共 20 条

[1] GLIAL AND NEURONAL FORMS OF THE VOLTAGE-DEPENDENT SODIUM-CHANNEL - CHARACTERISTICS AND CELL-TYPE DISTRIBUTION [J].