Tomato phospholipid hydroperoxide glutathione peroxidase inhibits cell death induced by Bax and oxidative stresses in yeast and plants

被引:119
作者
Chen, SR
Vaghchhipawala, Z
Li, W
Asard, H
Dickman, MB [1 ]
机构
[1] Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA
[2] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA
关键词
D O I
10.1104/pp.103.038091
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Using a conditional life or death screen in yeast, we have isolated a tomato (Lycopersicon esculentum) gene encoding a phospholipid hydroperoxide glutathione peroxidase (LePHGPx). The protein displayed reduced glutathione-dependent phospholipid hydroperoxide peroxidase activity, but differs from counterpart mammalian enzymes that instead contain an active seleno-Cys. LePHGPx functioned as a cytoprotector in yeast (Saccharomyces cerevisiae), preventing Bax, hydrogen peroxide, and heat stress induced cell death, while also delaying yeast senescence. When tobacco (Nicotiana tabacum) leaves were exposed to lethal levels of salt and heat stress, features associated with mammalian apoptosis were observed. Importantly, transient expression of LePHGPx protected tobacco leaves from salt and heat stress and suppressed the apoptotic-like features. As has been reported, conditional expression of Bax was lethal in tobacco, resulting in tissue collapse and membrane permeability to Evans blue. When LePHGPx was coexpressed with Bax, little cell death and no vital staining were observed. Moreover, stable expression of LePHGPx in tobacco conferred protection against the fungal phytopathogen Botrytis cinerea. Taken together, our data indicated that LePHGPx can protect plant tissue from a variety of stresses. Moreover, functional screens in yeast are a viable tool for the identification of plant genes that regulate cell death.
引用
收藏
页码:1630 / 1641
页数:12
相关论文
共 62 条
  • [51] Selenocysteine
    Stadtman, TC
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1996, 65 : 83 - 100
  • [52] A functional cloning strategy, based on a binary PVX-expression vector, to isolate HR-inducing cDNAs of plant pathogens
    Takken, FLW
    Luderer, R
    Gabriëls, SHEJ
    Westerink, N
    Lu, R
    de Wit, PJGM
    Joosten, MHAJ
    [J]. PLANT JOURNAL, 2000, 24 (02) : 275 - 283
  • [53] Oligomerized Ced-4 kills budding yeast through a caspase-independent mechanism
    Tao, WK
    Walke, DW
    Morgan, JI
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 260 (03) : 799 - 805
  • [54] Tiedemann A. V., 1997, Physiological and Molecular Plant Pathology, V50, P151, DOI 10.1006/pmpp.1996.0076
  • [55] THE SELENOENZYME PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE
    URSINI, F
    MAIORINO, M
    GREGOLIN, C
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1985, 839 (01) : 62 - 70
  • [56] PREVENTION OF PROGRAMMED CELL-DEATH IN CAENORHABDITIS-ELEGANS BY HUMAN BCL-2
    VAUX, DL
    WEISSMAN, IL
    KIM, SK
    [J]. SCIENCE, 1992, 258 (5090) : 1955 - 1957
  • [57] The molecular biology of apoptosis
    Vaux, DL
    Strasser, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (06) : 2239 - 2244
  • [58] Selection of mutant CHO cells with constitutive activation of the HIF system and inactivation of the von Hippel-Lindau tumor suppressor
    Vaux, EC
    Wood, SM
    Cockman, ME
    Nicholls, LG
    Yeates, KM
    Pugh, CW
    Maxwell, PH
    Ratcliffe, PJ
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (47) : 44323 - 44330
  • [59] Fumonisins and Alternaria alternata lycopersici toxins: Sphinganine analog mycotoxins induce apoptosis in monkey kidney cells
    Wang, H
    Jones, C
    CiacciZanella, J
    Holt, T
    Gilchrist, DG
    Dickman, MB
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (08) : 3461 - 3465
  • [60] A SIMPLE METHOD FOR DETECTION OF VIRAL SATELLITE RNAS IN SMALL PLANT-TISSUE SAMPLES
    WHITE, JL
    KAPER, JM
    [J]. JOURNAL OF VIROLOGICAL METHODS, 1989, 23 (02) : 83 - 93