Performance evaluation of the automated NucliSens easyMAG nucleic acid extraction platform in comparison with QIAamp Mini kit from clinical specimens

被引：19

作者：

Francesca, Perandin ^{[1
]}

Patrizia, Pollara C. ^{[2
]}

Franco, Gargiulo ^{[2
]}

Carlo, Bonfanti

Manca, Nino

机构：

[1] Univ Brescia, Dept Appl & Expt Med, Dept Microbiol, Microbiol Sect, I-25123 Brescia, Italy

[2] Spedali Civil Brescia, UO Microbiol, I-25123 Brescia, Italy

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2009年 / 64卷 / 02期

关键词：

CMV; EBV; HIV; Automated extraction; Realtime PCR; RNA EXTRACTION; DNA; PCR; VIRUS;

D O I：

10.1016/j.diagmicrobio.2009.02.013

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The performance of the NucliSens easyMAG platform for the extraction of nucleic acid from different clinical specimens was compared with a manual procedure. A total of 308 specimens were analyzed: 209 plasma samples collected for virus detection and quantification of cytomegalovirus (CMV) and Epstein-Barr virus (EBV) (n = 70), and 29 for HIV genotyping for drug resistance. Linearity of extraction was tested on dilution series of CMV and EBV the correlation coefficient (R-2) for standard curves based on repeated extraction runs was 0.99 for CMV and EBV. Inter- and intrarun variability was in accordance with previous studies, and the correlation between automated and manual extraction was very high. The concordant results were 95.7% for CMV and 100% for EBV The results of sequence analysis for HIV drug resistance showed a concordance in 24 of 29 specimens. The NucliSens easyMAG is extremely easy to perform, is automated, and resulted in a strong reduction of hands-on time compared with manual protocol. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：158 / 165

页数：8

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