SPI-C and STAT6 can cooperate to stimulate IgE germline transcription

被引：8

作者：

Carlsson, Robert ^{[1
]}

Thorell, Kaisa ^{[1
]}

Liberg, David ^{[1
]}

Leanderson, Tomas ^{[1
]}

机构：

[1] Lund Univ, Immunol Grp, S-22184 Lund, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 04期

关键词：

transcription; ETS proteins; STAT proteins;

D O I：

10.1016/j.bbrc.2006.04.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SPI-C is a novel ETS protein that is expressed in B lymphocytes. No target gene for SPI-C has so far been defined. We have performed a yeast two-hybrid screen using SPI-C as bait in order to further analyze the functional role of this orphan transcription factor. We found that SPI-C interacted specifically with the C-terminus of STAT6 in yeast. By co-immunoprecipitation in transfected COS7 cells the physical interaction between SPI-C and STAT6 was confirmed. Furthermore, this protein-protein interaction is functional since we could demonstrate that SPI-C and STAT6 stimulated IL4 induced 1 epsilon transcription synergistically but only when both proteins bound to DNA. Thus, a protein interaction between SPI-C and STAT6 is the basis for a novel mechanism for regulation of IL4 induced gene expression. (c) 2006 Elsevier Inc. All rights reserved.

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收藏

页码：1155 / 1160

页数：6

相关论文

共 15 条

[1] Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development [J].

Bemark, M ;

Mårtensson, A ;

Liberg, D ;

Leanderson, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (15) :10259-10267

[2] SPI-C, a PU-box binding ETS protein expressed temporarily during B-cell development and in macrophages, contains an acidic transactivation domain located to the N-terminus [J].

Carlsson, R ;

Persson, C ;

Leanderson, T .

MOLECULAR IMMUNOLOGY, 2003, 39 (16) :1035-1043

[3] Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C [J].

Carlsson, R ;

Hjalmarsson, A ;

Liberg, D ;

Persson, C ;

Leanderson, T .

GENE, 2002, 299 (1-2) :271-278

[4]

Gietz RD, 2002, METHOD ENZYMOL, V350, P87

[5] Pub, a novel PU.1 binding protein, regulates the transcriptional activity of PU.1 [J].

Hirose, S ;

Nishizumi, H ;

Sakano, H .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 311 (02) :351-360

[6]

Klemsz MJ, 1996, MOL CELL BIOL, V16, P390

[7] Oct proteins are qualitative rather than quantitative regulators of κ transcription [J].

Liberg, D ;

Sigvardsson, M ;

Leanderson, T .

MOLECULAR IMMUNOLOGY, 1997, 34 (14) :979-986

[8] Comparison of the transactivation domains of Stat5 and Stat6 in lymphoid cells and mammary epithelial cells [J].

Moriggl, R ;

Berchtold, S ;

Friedrich, K ;

Standke, GJR ;

Kammer, W ;

Heim, M ;

Wissler, M ;

Stocklin, E ;

Gouilleux, F ;

Groner, B .

MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (07) :3663-3678

[9] Identification of Smad7, a TGF beta-inducible antagonist of TGF-beta signalling [J].

Nakao, A ;

Afrakhte, M ;

Moren, A ;

Nakayama, T ;

Christian, JL ;

Heuchel, R ;

Itoh, S ;

Kawabata, N ;

Heldin, NE ;

Heldin, CH ;

tenDijke, P .

NATURE, 1997, 389 (6651) :631-635

[10] PU.1 is required for transcriptional activation of the Stat6 response element in the Igε promoter [J].

Pesu, M ;

Aittomäki, S ;

Välineva, T ;

Silvennoinen, O .

EUROPEAN JOURNAL OF IMMUNOLOGY, 2003, 33 (06) :1727-1735