SPI-C and STAT6 can cooperate to stimulate IgE germline transcription

被引：8

作者：

Carlsson, Robert ^{[1
]}

Thorell, Kaisa ^{[1
]}

Liberg, David ^{[1
]}

Leanderson, Tomas ^{[1
]}

机构：

[1] Lund Univ, Immunol Grp, S-22184 Lund, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2006年 / 344卷 / 04期

关键词：

transcription; ETS proteins; STAT proteins;

D O I：

10.1016/j.bbrc.2006.04.026

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SPI-C is a novel ETS protein that is expressed in B lymphocytes. No target gene for SPI-C has so far been defined. We have performed a yeast two-hybrid screen using SPI-C as bait in order to further analyze the functional role of this orphan transcription factor. We found that SPI-C interacted specifically with the C-terminus of STAT6 in yeast. By co-immunoprecipitation in transfected COS7 cells the physical interaction between SPI-C and STAT6 was confirmed. Furthermore, this protein-protein interaction is functional since we could demonstrate that SPI-C and STAT6 stimulated IL4 induced 1 epsilon transcription synergistically but only when both proteins bound to DNA. Thus, a protein interaction between SPI-C and STAT6 is the basis for a novel mechanism for regulation of IL4 induced gene expression. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：1155 / 1160

页数：6

共 15 条

[11] EFFECT OF PU.1 PHOSPHORYLATION ON INTERACTION WITH NF-EM5 AND TRANSCRIPTIONAL ACTIVATION [J].