Easy to optimize: Dynamic combinatorial libraries of metal-dye complexes as flexible sensors for tripeptides

被引：47

作者：

Buryak, Andrey ^{[1
]}

Severin, Kay ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, Inst Sci & Ingn Chim, Lausanne, Switzerland

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2006年 / 8卷 / 04期

关键词：

D O I：

10.1021/cc060028s

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A dynamic combinatorial library (DCL) of dye complexes of Cu2+ and Ni2+ was used to sense the sequence isomeric tripeptides His-Gly-Gly, Gly-His-Gly, and Gly-Gly-His. The DCL sensor was obtained by mixing buffered aqueous solution of three commercially available dyes together with CuCl2 and NiCl2. The addition of the peptide analytes resulted in characteristic changes in the UV-vis spectrum of the mixture, which allowed the identification of the peptide. Due to the modular nature of the sensor, it was possible to optimize the sensor by variation of the amounts and ratio of its constituent building blocks. The composition of the best sensor was found to vary substantially, depending on the sensing problem to be addressed. Whereas a Cu2+/Ni2+ ratio of 1:3 gave the best differentiation for Gly-His-Gly and Gly-Gly-His, a sensor containing exclusively Cu2+ was found to provide the best discrimination between His-Gly-Gly and Gly-Gly-His.

引用

页码：540 / 543

页数：4

共 41 条

[11] Adaptive behavior of dynamic combinatorial libraries generated by assembly of different building blocks [J].

Grote, Z ;

Scopelliti, R ;

Severin, K .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (32) :3821-3825

[12] Self-assembly of tetra- and hexanuclear circular helicates [J].

Hasenknopf, B ;

Lehn, JM ;

Boumediene, N ;

DupontGervais, A ;

VanDorsselaer, A ;

Kneisel, B ;

Fenske, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (45) :10956-10962

[13] Self-assembly of a circular double helicate [J].

Hasenknopf, B ;

Lehn, JM ;

Kneisel, BO ;

Baum, G ;

Fenske, D .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH, 1996, 35 (16) :1838-1840

[14] DYNAMIC MOLECULAR RECOGNITION IN A MULTIFUNCTIONAL PORPHYRIN AND A UBIQUINONE ANALOG [J].

HAYASHI, T ;

ASAI, T ;

HOKAZONO, H ;

OGOSHI, H .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (25) :12210-12211

[15] Virtual combinatorial libraries: Dynamic generation of molecular and supramolecular diversity by self-assembly [J].

Huc, I ;

Lehn, JM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2106-2110

[16] EPISELECTION - NOVEL K-I-SIMILAR-TO NANOMOLAR INHIBITORS OF SERINE PROTEASES SELECTED BY BINDING OR CHEMISTRY ON AN ENZYME SURFACE [J].

KATZ, BA ;

FINERMOORE, J ;

MORTEZAEI, R ;

RICH, DH ;

STROUD, RM .

BIOCHEMISTRY, 1995, 34 (26) :8264-8280

[17] Generation of novel DNA-binding compounds by selection and amplification from self-assembled combinatorial libraries [J].

Klekota, B ;

Hammond, MH ;

Miller, BL .

TETRAHEDRON LETTERS, 1997, 38 (50) :8639-8642

[18] Specific structure-stability relations in metallopeptides [J].

Kozlowski, H ;

Bal, W ;

Dyba, M ;

Kowalik-Jankowska, T .

COORDINATION CHEMISTRY REVIEWS, 1999, 184 :319-346

[19] A luminescent receptor with affinity for N-terminal histidine in peptides in aqueous solution [J].

Kruppa, M ;

Mandl, C ;

Miltschitzky, S ;

König, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2005, 127 (10) :3362-3365

[20]

Lavigne JJ, 2001, ANGEW CHEM INT EDIT, V40, P3118, DOI 10.1002/1521-3773(20010903)40:17<3118::AID-ANIE3118>3.0.CO

← 1 2 3 4 5 →