Spindle Assembly Checkpoint Protein Dynamics Reveal Conserved and Unsuspected Roles in Plant Cell Division

被引:61
作者
Caillaud, Marie-Cecile [1 ,2 ,3 ]
Paganelli, Laetitia [1 ,2 ,3 ]
Lecomte, Philippe [1 ,2 ,3 ]
Deslandes, Laurent [4 ]
Quentin, Michael [1 ,2 ,3 ]
Pecrix, Yann [5 ]
Le Bris, Manuel [5 ]
Marfaing, Nicolas [1 ,2 ,3 ]
Abad, Pierre [1 ,2 ,3 ]
Favery, Bruno [1 ,2 ,3 ]
机构
[1] INRA, Unite Mixte Rech 1301, Sophia Antipolis, France
[2] CNRS, Unite Mixte Rech 6243, Sophia Antipolis, France
[3] Univ Nice Sophia Antipolis, Unite Mixte Rech 1301, Sophia Antipolis, France
[4] CNRS, INRA, Unite Mixte Rech 2594, Castanet Tolosan, France
[5] Univ Paul Cezanne, IMEP, Unite Mixte Rech, CNRS,Inst Rech Dev 6116, Marseille, France
来源
PLOS ONE | 2009年 / 4卷 / 08期
关键词
D O I
10.1371/journal.pone.0006757
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: In eukaryotes, the spindle assembly checkpoint ( SAC) ensures that chromosomes undergoing mitosis do not segregate until they are properly attached to the microtubules of the spindle. Methodology/Principal Findings: We investigated the mechanism underlying this surveillance mechanism in plants, by characterising the orthogolous SAC proteins BUBR1, BUB3 and MAD2 from Arabidopsis. We showed that the cell cycle-regulated BUBR1, BUB3.1 and MAD2 proteins interacted physically with each other. Furthermore, BUBR1 and MAD2 interacted specifically at chromocenters. Following SAC activation by global defects in spindle assembly, these three interacting partners localised to unattached kinetochores. In addition, in cases of 'wait anaphase', plant SAC proteins were associated with both kinetochores and kinetochore microtubules. Unexpectedly, BUB3.1 was also found in the phragmoplast midline during the final step of cell division in plants. Conclusions/Significance: We conclude that plant BUBR1, BUB3.1 and MAD2 proteins may have the SAC protein functions conserved from yeast to humans. The association of BUB3.1 with both unattached kinetochore and phragmoplast suggests that in plant, BUB3.1 may have other roles beyond the spindle assembly checkpoint itself. Finally, this study of the SAC dynamics pinpoints uncharacterised roles of this surveillance mechanism in plant cell division.
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页数:9
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