Adaptation of the yeast Yarrowia lipolytica to ethanol

被引：7

作者：

Biryukova, E. N. ^{[1
]}

Arinbasarova, A. Yu. ^{[1
]}

Medentsev, A. G. ^{[1
]}

机构：

[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Oblast, Russia

来源：

MICROBIOLOGY | 2009年 / 78卷 / 02期

关键词：

Yarrowia lipolytica; stress; adaptation; ethanol; respiratory activity; inhibitor analysis; alternative oxidase; cAMP; NAD(+)-dependent alcohol dehydrogenase; SACCHAROMYCES-CEREVISIAE; HEAT-SHOCK; CATALASE-T; CTT1; GENE; GROWTH; TRANSCRIPTION; FERMENTATION; ALCOHOLS;

D O I：

10.1134/S0026261709020039

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

Resistance of the yeast Yarrowia lipolytica to ethanol stress was studied under different ethanol concentrations and treatment duration. Cell viability was shown to increase in the variants including preliminary treatment with small doses of ethanol, oxidants, or soft thermal exposure. The study of the respiratory activity under ethanol stress revealed the involvement of an alternative cyanide-resistant oxidase in the adaptive response of the cells. The level of intracellular cAMP decreased in response to the action of ethanol, which correlated with increased activity of the antioxidant systems (catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, glutathione reductase) and NAD(+)-dependent alcohol dehydrogenase.

引用

页码：154 / 159

页数：6

共 21 条

[1]

ALCOHOL-DEHYDROGENASE (ADH) IN YEASTS .2. NAD+-DEPENDENT AND NADP+-DEPENDENT ALCOHOL DEHYDROGENASES IN SACCHAROMYCOPSIS-LIPOLYTICA [J].