Imaging the Glycosylation State of Cell Surface Glycoproteins by Two-Photon Fluorescence Lifetime Imaging Microscopy

被引：78

作者：

Belardi, Brian ^{[1
,2
]}

de la Zerda, Adam ^{[1
,2
,3
]}

Spiciarich, David R. ^{[1
,2
]}

Maund, Sophia L. ^{[4
]}

Peehl, Donna M. ^{[4
]}

Bertozzi, Carolyn R. ^{[1
,2
]}

机构：

[1] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Chem, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[3] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

[4] Stanford Univ, Sch Med, Dept Urol, Stanford, CA 94305 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 52期

关键词：

bioorthogonal chemistry; fluorescence; glycosylation; metabolic incorporation; prostate cancer; CANCER; INTEGRINS; GLYCANS;

D O I：

10.1002/anie.201307512

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Glycoproteins in focus: Metabolic labeling of glycans with azido sugars (see picture) in combination with two-photon fluorescence lifetime imaging microscopy enables the visualization of specific glycoforms of endogenous proteins. This method can be utilized to detect glycosylated proteins in both cell culture and intact human tissue slices. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：14045 / 14049

页数：5

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