SDPR induces membrane curvature and functions in the formation of caveolae

被引：205

作者：

Hansen, Carsten G. ^{[1
]}

Bright, Nicholas A. ^{[2
]}

Howard, Gillian ^{[1
]}

Nichols, Benjamin J. ^{[1
]}

机构：

[1] MRC LMB, Cambridge CB2 0QH, England

[2] Univ Cambridge, Cambridge Inst Med Res, Addenbrookes Hosp, Cambridge CB2 0XY, England

来源：

NATURE CELL BIOLOGY | 2009年 / 11卷 / 07期

关键词：

DEPRIVATION-RESPONSE-GENE; TRANSCRIPT RELEASE FACTOR; COILED-COIL PROTEIN; SHIGA TOXIN; INDEPENDENT ENDOCYTOSIS; SERUM STARVATION; BINDING PROTEIN; POLYMERASE-I; PATHWAYS; CELLS;

D O I：

10.1038/ncb1887

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Caveolae are plasma membrane invaginations with a characteristic flask-shaped morphology. They function in diverse cellular processes, including endocytosis. The mechanism by which caveolae are generated is not fully understood, but both caveolin proteins and PTRF (polymerase I and transcript release factor, also known as cavin) are important. Here we show that loss of SDPR (serum deprivation protein response) causes loss of caveolae. SDPR binds directly to PTRF and recruits PTRF to caveolar membranes. Overexpression of SDPR, unlike PTRF, induces deformation of caveolae and extensive tubulation of the plasma membrane. The B-subunit of Shiga toxin (STB) also induces membrane tubulation and these membrane tubes also originate from caveolae. STB colocalizes extensively with both SDPR and caveolin 1. Loss of caveolae reduces the propensity of STB to induce membrane tubulation. We conclude that SDPR is a membrane-curvature-inducing component of caveolae, and that STB-induced membrane tubulation is facilitated by caveolae.

引用

页码：807 / U60

页数：17

共 39 条

[1] Vectorial proteomics reveal targeting, phosphorylation and specific fragmentation of polymerase I and transcript release factor (PTRF) at the surface of caveolae in human adipocytes [J].