Isolation and characterization of chitinase from a flake-chitin degrading marine bacterium, Aeromonas hydrophila H-2330

被引：37

作者：

Hiraga, K ^{[1
]}

Shou, L ^{[1
]}

Kitazawa, M ^{[1
]}

Takahashi, S ^{[1
]}

Shimada, M ^{[1
]}

Sato, R ^{[1
]}

Oda, K ^{[1
]}

机构：

[1] CENT RES INST, TSUKUBA, IBARAKI 30042, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 01期

关键词：

chitinase; flake-chitin; purification; marine bacterium; Aeromonas hydrophila;

D O I：

10.1271/bbb.61.174

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A flake-chitin degrading marine bacterium was isolated and identified as Aeromonas hydrophila. This strain secreted five chitinases and an beta-N-acetylglucosaminidase. The main chitinase (Chi-A) was purified and characterized, The optimum pH of Chi-A was 5-8, and the activity was inhibited by Hg2+ and Fe3+. Chi-A Has different from chitinases of other Aeromonas species with respect to molecular weight (62,000) and insensitivity to monoiodoacetate. The amino-terminal amino acid sequence showed extensive similarity with chitinases from Gram-negative bacteria.

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页码：174 / 176

页数：3

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