Lead modulation of the neuronal nicotinic acetylcholine receptor in PC12 cells

被引：11

作者：

Nagata, K ^{[1
]}

Huang, CS ^{[1
]}

Song, JH ^{[1
]}

Narahashi, T ^{[1
]}

机构：

[1] NORTHWESTERN UNIV,SCH MED,DEPT MOL PHARMACOL & BIOL CHEM,CHICAGO,IL 60611

来源：

BRAIN RESEARCH | 1997年 / 754卷 / 1-2期

关键词：

lead; heavy metal; nicotinic acetylcholine receptor; ion channel; PC12; cell;

D O I：

10.1016/S0006-8993(97)00037-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Lead is known to modulate several ligand- and voltage-gated ion channels, including the nicotinic acetylcholine receptor (AChR) channel. We examined the effects of lead on the nicotinic AChR in rat clonal phaeochromocytoma PC12 cells using whole-cell and single-channel patch-clamp techniques to clarify the detailed mechanism of action. Lead suppressed acetylcholine-induced currents in a dose-dependent manner with an EC50 value of 37 mu M and a Hill coefficient of 0.82. At the single-channel level, 1-10 mu M lead shortened the opening and burst durations, and increased the duration of mean closed time. The open probability was significantly decreased by lead. These changes of single-channel kinetics result in a significant decrease in the total charge carried through the open AChR channels explaining the suppressive effect of lead on acetylcholine-induced whole-cell currents. (C) 1997 Elsevier Science B.V.

引用

页码：21 / 27

页数：7

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