PIN: An associated protein inhibitor of neuronal nitric oxide synthase

被引：411

作者：

Jaffrey, SR

Snyder, SH

机构：

[1] JOHNS HOPKINS UNIV,SCH MED,DEPT NEUROSCI,BALTIMORE,MD 21205

[2] JOHNS HOPKINS UNIV,SCH MED,DEPT PHARMACOL & MOL SCI,BALTIMORE,MD 21205

[3] JOHNS HOPKINS UNIV,SCH MED,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21205

来源：

SCIENCE | 1996年 / 274卷 / 5288期

关键词：

D O I：

10.1126/science.274.5288.774

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The neurotransmitter functions of nitric oxide are dependent on dynamic regulation of its biosynthetic enzyme, neuronal nitric oxide synthase (nNOS), By means of a yeast two-hybrid screen, a 10-kilodalton protein was identified that physically interacts with and inhibits the activity of nNOS. This inhibitor, designated PIN, appears to be one of the most conserved proteins in nature, showing 92 percent amino acid identity with the nematode and rat homologs,Binding of PIN destabilizes the nNOS dimer, a conformation necessary for activity. These results suggest that PIN may regulate numerous biological processes through its effects on nitric oxide synthase activity.

引用

页码：774 / 777

页数：4

共 35 条

[1] 14-3-3 PROTEINS - A HIGHLY CONSERVED, WIDESPREAD FAMILY OF EUKARYOTIC PROTEINS
AITKEN, A
COLLINGE, DB
VANHEUSDEN, BPH
ISOBE, T
ROSEBOOM, PH
ROSENFELD, G
SOLL, J
[J]. TRENDS IN BIOCHEMICAL SCIENCES, 1992, 17 (12) : 498 - 501
[2] ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999
[3] APOPTOSIS AND NECROSIS - 2 DISTINCT EVENTS INDUCED, RESPECTIVELY, BY MILD AND INTENSE INSULTS WITH N-METHYL-D-ASPARTATE OR NITRIC-OXIDE SUPEROXIDE IN CORTICAL CELL-CULTURES
BONFOCO, E
KRAINC, D
ANKARCRONA, M
NICOTERA, P
LIPTON, SA
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (16) : 7162 - 7166
[4] NITRIC-OXIDE MEDIATES GLUTAMATE-LINKED ENHANCEMENT OF CGMP LEVELS IN THE CEREBELLUM
BREDT, DS
SNYDER, SH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) : 9030 - 9033
[5] CLONING AND EXPRESSION OF 2 BRAIN-SPECIFIC INWARDLY RECTIFYING POTASSIUM CHANNELS
BREDT, DS
WANG, TL
COHEN, NA
GUGGINO, WB
SNYDER, SH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (15) : 6753 - 6757
[6] ISOLATION OF NITRIC-OXIDE SYNTHETASE, A CALMODULIN-REQUIRING ENZYME
BREDT, DS
SNYDER, SH
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (02) : 682 - 685
[7] Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha 1-syntrophin mediated by PDZ domains
Brenman, JE
Chao, DS
Gee, SH
McGee, AW
Craven, SE
Santillano, DR
Wu, ZQ
Huang, F
Xia, HH
Peters, MF
Froehner, SC
Bredt, DS
[J]. CELL, 1996, 84 (05) : 757 - 767
[8] NITRIC-OXIDE SYNTHASE COMPLEXED WITH DYSTROPHIN AND ABSENT FROM SKELETAL-MUSCLE SARCOLEMMA IN DUCHENNE MUSCULAR-DYSTROPHY
BRENMAN, JE
CHAO, DS
XIA, HH
ALDAPE, K
BREDT, DS
[J]. CELL, 1995, 82 (05) : 743 - 752
[9] PROTEIN-INTERACTION CLONING IN YEAST - IDENTIFICATION OF MAMMALIAN PROTEINS THAT REACT WITH THE LEUCINE ZIPPER OF JUN
CHEVRAY, PM
NATHANS, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (13) : 5789 - 5793
[10] Dick T, 1996, MOL CELL BIOL, V16, P1966

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