5′-end SAGE for the analysis of transcriptional start sites

被引：83

作者：

Hashimoto, S

Suzuki, Y

Kasai, Y

Morohoshi, K

Yamada, T

Sese, J

Morishita, S

Sugano, S

Matsushima, K

机构：

[1] Univ Tokyo, Grad Sch Frontier Sci, Sch Med, Dept Mol Prevent Med,Bunkyo Ku, Tokyo 1130033, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Inst Med Sci, Dept Virol,Bunkyo Ku, Tokyo 1130033, Japan

[3] Univ Tokyo, Grad Sch Frontier Sci, Dept Computat Biol, Bunkyo Ku, Tokyo 1130033, Japan

来源：

NATURE BIOTECHNOLOGY | 2004年 / 22卷 / 09期

关键词：

D O I：

10.1038/nbt998

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Identification of the mRNA start site is essential in establishing the full-length cDNA sequence of a gene and analyzing its promoter region, which regulates gene expression. Here we describe the development of a 5'-end serial analysis of gene expression (5 SAGE) that can be used to globally identify transcriptional start sites and the frequency of individual mRNAs. Of the 25,684 5' SAGE tags in the HEK293 human cell library, 19,893 matched to the human genome. Among 15,448 tags in one locus of the genome, 85.8%-96.1% of the 5 SAGE tags were assigned within -500 to +200 nt of mRNA start sites using the RefSeq, UniGene and DBTSS databases. This technique should facilitate 5'-end transcriptome analysis in a variety of cells and tissues.

引用

页码：1146 / 1149

页数：4

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