Cationic oligopeptide-mediated delivery of dsRNA for post-transcriptional gene silencing in plant cells

被引：79

作者：

Unnamalai, N ^{[1
]}

Kang, BG ^{[1
]}

Lee, WS ^{[1
]}

机构：

[1] Sungkyunkwan Univ, Dept Biol Sci, Suwon 440746, South Korea

来源：

FEBS LETTERS | 2004年 / 566卷 / 1-3期

关键词：

polyarginine; double-stranded RNA; post-transcriptional gene silencing; RNase protection assay;

D O I：

10.1016/j.febslet.2004.04.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used cationic oligopeptide polyarginine-12mer (POA) to deliver double-stranded RNA (dsRNA), prepared in vitro, to tobacco (Nicotiana tabacum) suspension cells. POA interacts electrostatically with dsRNA to form a complex. When dsRNA for the GUS or NPTII gene was delivered into cells carrying the same genes, the corresponding mRNA was degraded. Using RNase protection assay we were able to detect 21-bp small interfering RNA in dsRNA/POA-treated cells. These results demonstrate that POA can be used to deliver dsRNA to induce post-transcriptional gene silencing in plant cells. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：307 / 310

页数：4

共 30 条

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