Investigating the role of DNA damage in tobacco smoking-induced spine degeneration

被引：114

作者：

Nasto, Luigi A. ^{[1
,2
]}

Ngo, Kevin ^{[1
]}

Leme, Adriana S. ^{[3
]}

Robinson, Andria R. ^{[4
]}

Dong, Qing ^{[1
]}

Roughley, Peter ^{[5
]}

Usas, Arvydas ^{[6
]}

Sowa, Gwendolyn A. ^{[1
,7
]}

Pola, Enrico ^{[2
]}

Kang, James ^{[1
]}

Niedernhofer, Laura J. ^{[4
,8
]}

Shapiro, Steven ^{[3
]}

Vo, Nam V. ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Med, Dept Orthopaed Surg, Ferguson Lab Orthopaed Res, Pittsburgh, PA 15261 USA

[2] Univ Cattolica Sacro Cuore, Dept Orthopaed Surg, Sch Med, A Gemelli Univ Hosp, I-00168 Rome, Italy

[3] Univ Pittsburgh, Sch Med, Dept Med, Pittsburgh, PA 15213 USA

[4] Univ Pittsburgh, Dept Microbiol & Mol Genet, Sch Med, Pittsburgh, PA 15219 USA

[5] Shriners Hosp Children, McGill Scoliosis & Spine Grp, Montreal, PQ H3G 1A6, Canada

[6] UPMC, Dept Orthopaed Surg, Stem Cell Res Ctr, Pittsburgh, PA 15261 USA

[7] Univ Pittsburgh, Sch Med, Dept Phys Med & Rehabil, Pittsburgh, PA 15261 USA

[8] Scripps Res Inst, Dept Metab & Aging, Jupiter, FL 33458 USA

来源：

SPINE JOURNAL | 2014年 / 14卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Tobacco smoking; Intervertebral disc degeneration; Matrix proteoglycans; Aggrecan; Matrix metalloproteinases; DNA damage repair; LOW-BACK-PAIN; LUMBAR DISC DEGENERATION; INTERVERTEBRAL DISC; CIGARETTE-SMOKING; MOUSE MODEL; MAGNETIC-RESONANCE; NUCLEUS PULPOSUS; CORE PROTEIN; MURINE MODEL; RISK-FACTORS;

D O I：

10.1016/j.spinee.2013.08.034

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

100204 [神经病学];

摘要：

BACKGROUND CONTEXT: Tobacco smoking is a key risk factor for spine degeneration. However, the underlying mechanism by which smoking induces degeneration is not known. Recent studies implicate DNA damage as a cause of spine and intervertebral disc degeneration. Because tobacco smoke contains many genotoxins, we hypothesized that tobacco smoking promotes spine degeneration by inducing cellular DNA damage. PURPOSE: To determine if DNA damage plays a causal role in smoking-induced spine degeneration. STUDY DESIGN: To compare the effect of chronic tobacco smoke inhalation on intervertebral disc and vertebral bone in normal and DNA repair-deficient mice to determine the contribution of DNA damage to degenerative changes. METHODS: Two-month-old wild-type (C57BL/6) and DNA repair-deficient Ercc1(-/Delta) mice were exposed to tobacco smoke by direct inhalation (4 cigarettes/day, 5 days/week for 7 weeks) to model first-hand smoking in humans. Total disc proteoglycan (PG) content (1,9-dimethylmethylene blue assay), PG synthesis (35 S-sulfate incorporation assay), aggrecan proteolysis (immunoblotting analysis), and vertebral bone morphology (microcomputed tomography) were measured. RESULTS: Exposure of wild-type mice to tobacco smoke led to a 19% increase in vertebral porosity and a 61% decrease in trabecular bone volume. Intervertebral discs of smoke-exposed animals also showed a 2.6-fold decrease in GAG content and an 8.1-fold decrease in new PG synthesis. These smoking-induced degenerative changes were similar but not worse in Ercc1(-/Delta) mice. CONCLUSIONS: Short-term exposure to high levels of primary tobacco smoke inhalation promotes degeneration of vertebral bone and discs. Disc degeneration is primarily driven by reduced synthesis of proteoglycans needed for vertebral cushioning. Degeneration was not exacerbated in congenic DNA repair-deficient mice, indicating that DNA damage per se does not have a significant causal role in driving smoke-induced spine degeneration. Published by Elsevier Inc.

引用

页码：416 / 423

页数：8

共 41 条

[1]

Genetic risk factors for lumbar disc disease [J].