Production of plant-specific tyramine derivatives by dual expression of tyramine N-hydroxycinnamoyltransferase and 4-coumarate:coenzyme A ligase in Escherichia coli

被引：6

作者：

Kang, Kiyoon ^{[1
]}

Park, Munyoung ^{[1
]}

Park, Sangkyu ^{[1
]}

Kim, Young Soon ^{[1
]}

Lee, Sungbeom ^{[1
]}

Lee, Seong-Gene ^{[1
,2
]}

Back, Kyoungwhan ^{[1
]}

机构：

[1] Chonnam Natl Univ, Dept Biotechnol, Interdisciplinary Program Bioenergy & Biomat, Biotechnol Res Inst,Grad Sch, Kwangju 500757, South Korea

[2] Virginia Tech, Blacksburg, VA 24061 USA

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 09期

关键词：

Dopamine derivatives; Dual expression; Feruloyltyramine; Feruloyldopamine; Escherichia coli; Tyramine derivatives; PEPPER TYRAMINE; TOBACCO-LEAVES; CLONING; CDNA; N-(HYDROXYCINNAMOYL)TRANSFERASE; FERULOYLTYRAMINE;

D O I：

10.1007/s10529-009-0032-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant-specific bioactive compounds including feruloyltyramine (FT), 4-coumaroyltyramine (CT), and caffeoyltyramine (CaT) were simultaneously produced in Escherichia coli by heterologous expression of two biosynthetic genes encoding 4-coumarate:coenzyme A ligase and tyramine N-hydroxycinnamoyltransferase (THT) cloned from Arabidopsis thaliana and pepper, respectively. Simultaneous supplementation of substrates to the recombinant E. coli resulted in the secretion of multiple tyramine derivatives into the medium at high yield: CT (189 mg l(-1)), FT (135 mg l(-1)), CaT (40 mg l(-1)). In addition, the recombinant E. coli also produced, albeit at low concentration, a range of dopamine derivatives such as feruloyldopamine due to THT's ability to accept dopamine as a substrate.

引用

页码：1469 / 1475

页数：7

共 23 条

[1] Cloning and characterization of a hydroxycinnamoyl-CoA :tyramine N-(hydroxycinnamoyl)transferase induced in response to UV-C and wounding from Capsicum annuum [J].