Spectral and 3-Dimensional Tracking of Single Gold Nanoparticles in Living Cells Studied by Rayleigh Light Scattering Microscopy

被引:48
作者
Louit, Guillaume [1 ]
Asahi, Tsuyoshi [1 ]
Tanaka, Go [1 ]
Uwada, Takayuki [3 ,4 ]
Masuhara, Hiroshi [2 ,3 ,4 ]
机构
[1] Osaka Univ, Dept Appl Phys, Suita, Osaka 5650871, Japan
[2] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan
[3] Natl Chiao Tung Univ, Dept Appl Chem, Hsinchu 30010, Taiwan
[4] Natl Chiao Tung Univ, Inst Mol Sci, Hsinchu 30010, Taiwan
基金
日本学术振兴会;
关键词
HIGHLY FLUORESCENT ANALOGS; PLASMON-RESONANCE; OPTICAL-PROPERTIES; SUBMICROSCOPIC PARTICLES; BIOLOGICAL APPLICATIONS; GENE-EXPRESSION; TRACER LABELS; IN-VIVO; DNA; SIZE;
D O I
10.1021/jp9018124
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Using a confocal Rayleigh light scattering microspectroscopy and imaging system, we followed and recorded the scattering spectra of single gold nanoparticles interacting with living mouse fibroblast cells, during the scale of I h. A white light continuum produced by a femtosecond laser was focused on gold nanoparticles with a diameter of 80 nm, while an incubation system ensured viability of the living cells. After reporting spectral characteristics of gold nanoparticles and their target fibroblasts, separately and in combination, tracking sequences of single nanoparticles in contact with a single cell are described. Through the study of three separate and representative cases, transitory as well as plasmon resonance band shifts, which can be linked to biological events, have been observed and are discussed here.
引用
收藏
页码:11766 / 11772
页数:7
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