A new syntaxin family member implicated in targeting of intracellular transport vesicles

被引：127

作者：

Bock, JB ^{[1
]}

Lin, RC ^{[1
]}

Scheller, RH ^{[1
]}

机构：

[1] STANFORD UNIV,MED CTR,HOWARD HUGHES MED INST,DEPT MOLEC & CELLULAR PHYSIOL,STANFORD,CA 94305

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 30期

关键词：

D O I：

10.1074/jbc.271.30.17961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite the central role vesicular trafficking occupies in protein targeting, the molecular coding of the trafficking signals and the mechanism of vesicle docking and fusion are just beginning to be understood. We report here the cloning and initial characterization of a new member of the syntaxin family of vesicular transport receptors, Syntaxin 6 is a 255-amino acid protein with two domains predicted to form coiled coils, as well as a carboxyl-terminal membrane anchor. Syntaxin 6 is broadly expressed and localizes in the region of the Golgi apparatus, In vitro binding studies established that syntaxin 6 binds to alpha-soluble NSF attachment protein (alpha-SNAP). The sequence homology, topology, localization, and alpha-SNAP binding suggest that syntaxin 6 is involved in intracellular vesicle trafficking.

引用

页码：17961 / 17965

页数：5

共 31 条

[1] SEQUENCE IDENTIFICATION OF 2,375 HUMAN BRAIN GENES [J].

ADAMS, MD ;

DUBNICK, M ;

KERLAVAGE, AR ;

MORENO, R ;

KELLEY, JM ;

UTTERBACK, TR ;

NAGLE, JW ;

FIELDS, C ;

VENTER, JC .

NATURE, 1992, 355 (6361) :632-634

[2] COMPLEMENTARY-DNA SEQUENCING - EXPRESSED SEQUENCE TAGS AND HUMAN GENOME PROJECT [J].

ADAMS, MD ;

KELLEY, JM ;

GOCAYNE, JD ;

DUBNICK, M ;

POLYMEROPOULOS, MH ;

XIAO, H ;

MERRIL, CR ;

WU, A ;

OLDE, B ;

MORENO, RF ;

KERLAVAGE, AR ;

MCCOMBIE, WR ;

VENTER, JC .

SCIENCE, 1991, 252 (5013) :1651-1656

[3]

ALTSCHUL SF, 1990, J MOL BIOL, V215, P403, DOI 10.1006/jmbi.1990.9999

[4] Novel syntaxin homologue, Pep12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast [J].

Becherer, KA ;

Rieder, SE ;

Emr, SD ;

Jones, EW .

MOLECULAR BIOLOGY OF THE CELL, 1996, 7 (04) :579-594

[5] THE SYNTAXIN FAMILY OF VESICULAR TRANSPORT RECEPTORS [J].

BENNETT, MK ;

GARCIAARRARAS, JE ;

ELFERINK, LA ;

PETERSON, K ;

FLEMING, AM ;

HAZUKA, CD ;

SCHELLER, RH .

CELL, 1993, 74 (05) :863-873

[6]

BENNETT MK, 1994, ANNU REV BIOCHEM, V63, P63, DOI 10.1146/annurev.biochem.63.1.63

[7] THE MOLECULAR MACHINERY FOR SECRETION IS CONSERVED FROM YEAST TO NEURONS [J].

BENNETT, MK ;

SCHELLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (07) :2559-2563

[8] SYNTAXIN - A SYNAPTIC PROTEIN IMPLICATED IN DOCKING OF SYNAPTIC VESICLES AT PRESYNAPTIC ACTIVE ZONES [J].

BENNETT, MK ;

CALAKOS, N ;

SCHELLER, RH .

SCIENCE, 1992, 257 (5067) :255-259

[9]

CHAPMAN ER, 1994, J BIOL CHEM, V269, P27427

[10] SNAPS, A FAMILY OF NSF ATTACHMENT PROTEINS INVOLVED IN INTRACELLULAR MEMBRANE-FUSION IN ANIMALS AND YEAST [J].

CLARY, DO ;

GRIFF, IC ;

ROTHMAN, JE .

CELL, 1990, 61 (04) :709-721

← 1 2 3 4 →