Regulation of chondrocytic phenotype by micro RNA 18a: Involvement of Ccn2/Ctgf as a major target gene

被引：72

作者：

Ohgawara, Toshihiro ^{[1
,2
]}

Kubota, Satoshi ^{[1
]}

Kawaki, Harumi ^{[1
]}

Kondo, Seiji ^{[1
]}

Eguchi, Takanori ^{[1
]}

Kurio, Naito ^{[2
]}

Aoyama, Eriko ^{[3
]}

Sasaki, Akira ^{[2
]}

Takigawa, Masaharu ^{[1
,3
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Biochem & Mol Dent, Okayama 7008525, Japan

[2] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Dept Oral & Maxillofacial Surg & Biopathol Sci, Okayama 7008525, Japan

[3] Okayama Univ, Sch Dent, Biodent Res Ctr, Okayama 7008525, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 06期

基金：

日本学术振兴会;

关键词：

Connective tissue growth factor; CCN family; Micro RNA; miR-18a; Chondrocyte; PROLIFERATION; CTGF/HCS24; DIFFERENTIATION; EXPRESSION; PRODUCT;

D O I：

10.1016/j.febslet.2009.02.025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We searched for miRNAs that were down-regulated in chondrocytic cells and predicted to target CCN2/connective tissue growth factor (CCN2/CTGF) that promotes endochondral ossification. Among them, expression of miR-18a was most strongly repressed in chondrocytic cells. Reporter gene analysis confirmed the functionality of an miR-18a target in the 3'-untranslated region of Ccn2 mRNA, which was predicted in silico. Indeed, introduction of miR-18a efficiently repressed the CCN2 production from chondrocytic cells. Finally, transfected miR-18a significantly repressed the mature chondrocytic phenotype. Our present study revealed a regulatory role for miR-18a in chondrocytic differentiation through CCN2. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：1006 / 1010

页数：5

共 17 条

[1] MicroRNAs in vertebrate development [J].