In vivo functional chronic imaging of a small animal model using optical-resolution photoacoustic microscopy

被引:64
作者
Hu, Song [1 ]
Maslov, Konstantin [1 ]
Wang, Lihong V. [1 ]
机构
[1] Washington Univ, Dept Biomed Engn, Opt Imaging Lab, St Louis, MO 63130 USA
基金
美国国家卫生研究院;
关键词
biochemistry; biomedical optical imaging; blood; blood vessels; ear; haemodynamics; laser applications in medicine; optical microscopy; oxygen; photoacoustic effect; surgery; INTRAVITAL MICROSCOPY; BLOOD-FLOW; BRAIN; MICROCIRCULATION; CAPILLARIES;
D O I
10.1118/1.3137572
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Optical-resolution photoacoustic microscopy (OR-PAM) has been validated as a valuable tool for label-free volumetric microvascular imaging. More importantly, the advantages of noninvasiveness and measurement consistency suggest the use of OR-PAM for chronic imaging of intact microcirculation. Here, such chronic imaging is demonstrated for the first time by monitoring the healing process of laser-induced microvascular lesions in a small animal model in vivo. The central part of a 1 mm by 1 mm region in a nude mouse ear was treated under a continuous-wave laser to create a microvascular lesion for chronic study. The region of interest was imaged before the laser treatment, immediately after the treatment, and throughout the healing process using both the authors' OR-PAM system and a commercial transmission-mode optical microscope. Three-dimensional microvascular morphology and blood oxygenation information were imaged simultaneously at capillary-level resolution. Transmission-mode optical microscopic images were acquired for comparison. OR-PAM has potential important applications in microcirculatory physiology or pathophysiology, tumor angiogenesis, laser microsurgery, and neuroscience.
引用
收藏
页码:2320 / 2323
页数:4
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