Evaluation of a new automated, standardized generic nucleic acid extraction method (Total nucleic acid isolation kit) used in combination with cytomegalovirus DNA quantification by COBAS AMPLICOR CMV MONITOR

被引：5

作者：

Gärtner, BC

Fischinger, JM

Litwicki, A

Roemer, K

Mueller-Lantzsch, N

机构：

[1] Univ Saarland, Inst Virol, D-66421 Homburg, Germany

[2] Univ Saarland, Dept Internal Med, D-66421 Homburg, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 08期

关键词：

D O I：

10.1128/JCM.42.8.3881-3882.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. The kit provides a reliable, standardized, and time-saving tool for DNA extraction.

引用

收藏

页码：3881 / 3882

页数：2

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