miR-210 promotes osteoblastic differentiation through inhibition of AcvR1b

被引：244

作者：

Mizuno, Yosuke ^{[2
]}

Tokuzawa, Yoshimi ^{[2
]}

Ninomiya, Yuichi ^{[2
]}

Yagi, Ken ^{[2
]}

Yatsuka-Kanesaki, Yukiko ^{[2
]}

Suda, Tatsuo ^{[2
]}

Fukuda, Toru ^{[3
]}

Katagiri, Takenobu ^{[3
]}

Kondoh, Yasumitsu ^{[4
]}

Amemiya, Tomoyuki ^{[4
]}

Tashiro, Hideo ^{[4
]}

Okazaki, Yasushi ^{[1
,2
]}

机构：

[1] Saitama Med Univ, Res Ctr Genom Med, Div Translat Res, Hidaka 3501241, Japan

[2] Saitama Med Univ, Res Ctr Genom Med, Div Funct Genom & Syst Med, Hidaka 3501241, Japan

[3] Saitama Med Univ, Res Ctr Genom Med, Div Pathophysiol, Hidaka 3501241, Japan

[4] RIKEN, Probing Technol Lab, Wako, Saitama 3510198, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 13期

关键词：

miR-210; Differentiation; Osteoblast; Activin A receptor type 1B; MICRORNAS; SUPPRESSOR; PROLIFERATION; ONCOGENES; REPRESSES; TARGETS; HYPOXIA;

D O I：

10.1016/j.febslet.2009.06.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although microRNAs (miRNAs) are involved in many biological processes, the mechanisms whereby miRNAs regulate osteoblastic differentiation are poorly understood. Here, we found that BMP-4-induced osteoblastic differentiation of bone marrow-derived ST2 stromal cells was promoted and repressed after transfection of sense and antisense miR-210, respectively. A reporter assay demonstrated that the activin A receptor type 1B (AcvR1b) gene was a target for miR-210. Furthermore, inhibition of transforming growth factor-beta (TGF-beta)/activin signaling in ST2 cells with SB431542 promoted osteoblastic differentiation. We conclude that miR-210 acts as a positive regulator of osteoblastic differentiation by inhibiting the TGF-beta/activin signaling pathway through inhibition of AcvR1b. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2263 / 2268

页数：6

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