Stereodifferentiation of chiral compounds using reversed-phase liquid chromatography coupled with capillary gas chromatography

被引：3

作者：

del Castillo, MLR ^{[1
]}

Caballero, EG ^{[1
]}

Herraiz, M ^{[1
]}

机构：

[1] CSIC, Inst Fermentac Ind, E-28006 Madrid, Spain

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2003年 / 41卷 / 01期

关键词：

D O I：

10.1093/chromsci/41.1.26

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for the enantiomeric quantitation of some chiral compounds via online coupling of reversed-phase liquid chromatography-gas chromatography. The evaluation of some variables affecting the experimentation (i.e., the packing material used in the interface, volume of the transferred fraction, desorption time, initial temperature of the interface, and purge time) makes it possible to optimize the recoveries obtained for some chiral terpenes and lactones using a capillary column of β-cyclodextrin dissolved in OV-1701. The proposed method allows the enantiomeric analysis of aqueous matrices obtaining relative standard deviations lower than 9% and detection limits ranging from 0.26-0.93 ppm for the investigated compounds.

引用

页码：26 / 30

页数：5

共 18 条

[1]

[Anonymous], 1991, ON LINE COUPLED LC G

[2]

Bertsch W, 2000, HRC-J HIGH RES CHROM, V23, P167

[3] Enantiomer analysis of chiral lactones in foods by on-line coupled reversed-phase liquid chromatography gas chromatography [J].