Rpi-vnt1.1, a Tm-22 Homolog from Solanum venturii, Confers Resistance to Potato Late Blight

被引:153
作者
Foster, Simon J. [1 ]
Park, Tae-Ho [1 ]
Pel, Mathieu [2 ]
Brigneti, Gianinna [1 ]
Sliwka, Jadwiga [3 ]
Jagger, Luke [1 ]
van der Vossen, Edwin [2 ]
Jones, Jonathan D. G. [1 ]
机构
[1] John Innes Ctr, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England
[2] Wageningen UR Plant Breeding, NL-6700 AJ Wageningen, Netherlands
[3] Res Ctr Mlochow, Plant Breeding & Acclimatizat Inst, PL-05831 Mlochow, Poland
基金
英国生物技术与生命科学研究理事会; 美国国家科学基金会;
关键词
BROAD-SPECTRUM RESISTANCE; RACE-SPECIFIC RESISTANCE; PHYTOPHTHORA-INFESTANS MONT; DISEASE-RESISTANCE; CHROMOSOME-IX; LYCOPERSICON-ESCULENTUM; R-GENE; HYPERSENSITIVE RESISTANCE; UNITED-STATES; AFLP MARKERS;
D O I
10.1094/MPMI-22-5-0589
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Despite the efforts of breeders and the extensive use of fungicide control measures, late blight still remains a major threat to potato cultivation worldwide. The introduction of genetic resistance into cultivated potato is considered a valuable method to achieve durable resistance to late blight. Here, we report the identification and cloning of Rpi-vnt1.1, a previously uncharacterized late-blight resistance gene from Solanum venturii. The gene was identified by a classical genetic and physical mapping approach and encodes a coiled-coil nucleotide-binding leucine-rich repeat protein with high similarity to Tm-22 from S. lycopersicum which confers resistance against Tomato mosaic virus. Transgenic potato and tomato plants carrying Rpi-vnt1.1 were shown to be resistant to Phytophthora infestans. Of 11 P. infestans isolates tested, only isolate EC1 from Ecuador was able to overcome Rpi-vnt1.1 and cause disease on the inoculated plants. Alleles of Rpi-vnt1.1 (Rpi-vnt1.2 and Rpi-vnt1.3) that differed by only a few nucleotides were found in other late-blight-resistant accessions of S. venturii. The late blight resistance gene Rpi-phu1 from S. phureja is shown here to be identical to Rpi-vnt1.1, suggesting either that this strong resistance gene has been maintained since a common ancestor, due to selection pressure for blight resistance, or that genetic exchange between S. venturii and S. phureja has occurred at some time. © 2009 The American Phytopathological Society.
引用
收藏
页码:589 / 600
页数:12
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