Trophic Effects of Dental Pulp Stem Cells on Schwann Cells in Peripheral Nerve Regeneration

被引：59

作者：

Yamamoto, Tsubasa ^{[1
,2
]}

Osako, Yohei ^{[1
]}

Ito, Masataka ^{[3
]}

Murakami, Masashi ^{[1
]}

Hayashi, Yuki ^{[1
,4
]}

Horibe, Hiroshi ^{[1
,2
]}

Iohara, Koichiro ^{[1
]}

Takeuchi, Norio ^{[1
,5
]}

Okui, Nobuyuki ^{[6
]}

Hirata, Hitoshi ^{[7
]}

Nakayama, Hidenori ^{[1
,2
]}

Kurita, Kenichi ^{[2
]}

Nakashima, Misako ^{[1
]}

机构：

[1] Natl Ctr Geriatr & Gerontol, Ctr Adv Med Dent & Oral Dis, Res Inst, Morioka, Obu, Japan

[2] Aichi Gakuin Univ, Sch Dent, Dept Oral & Maxillofacial Surg, Chikusa Ku, 1-100 Kusumoto Cho, Nagoya, Aichi 464, Japan

[3] Natl Def Med Coll, Dept Dev Anat & Regenerat Med, Tokorozawa, Saitama 359, Japan

[4] Aichi Gakuin Univ, Sch Dent, Dept Pediat Dent, Chikusa Ku, 1-100 Kusumoto Cho, Nagoya, Aichi 464, Japan

[5] Aichi Gakuin Univ, Sch Dent, Dept Endodont, Chikusa Ku, 1-100 Kusumoto Cho, Nagoya, Aichi 464, Japan

[6] Nagoya Ekisaikai Hosp, Dept Orthoped Surg & Rheumatol, Nakagawa Ku, Nagoya, Aichi, Japan

[7] Nagoya Univ, Dept Hand Surg, Grad Sch Med, Showa Ku, Nagoya, Aichi 4648601, Japan

来源：

CELL TRANSPLANTATION | 2016年 / 25卷 / 01期

关键词：

Mobilized dental pulp stem cells (MDPSCs); Peripheral nerve regeneration; Cell transplantation; Schwann cells; Trophic effect; Sciatic nerve; COLONY-STIMULATING FACTOR; SIDE POPULATION CELLS; BONE-MARROW; ANGIOGENESIS; INJURY; REPAIR; RATS; DIFFERENTIATION; MECHANISMS; ISCHEMIA;

D O I：

10.3727/096368915X688074

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Recently, mesenchymal stem cells have demonstrated a potential for neurotrophy and neurodifferentiation. We have recently isolated mobilized dental pulp stem cells (MDPSCs) using granulocyte-colony stimulating factor (G-CSF) gradient, which has high neurotrophic/angiogenic potential. The aim of this study is to investigate the effects of MDPSC transplantation on peripheral nerve regeneration. Effects of MDPSC transplantation were examined in a rat sciatic nerve defect model and compared with autografts and control conduits containing collagen scaffold. Effects of conditioned medium of MDPSCs were also evaluated in vitro. Transplantation of MDPSCs in the defect demonstrated regeneration of myelinated fibers, whose axons were significantly higher in density compared with those in autografts and control conduits only. Enhanced revascularization was also observed in the MDPSC transplants. The MDPSCs did not directly differentiate into Schwann cell phenotype; localization of these cells near Schwann cells induced several neurotrophic factors. Immunofluorescence labeling demonstrated reduced apoptosis and increased proliferation in resident Schwann cells in the MDPSC transplant compared with control conduits. These trophic effects of MDPSCs on proliferation, migration, and antiapoptosis in Schwann cells were further elucidated in vitro. The results demonstrate that MDPSCs promote axon regeneration through trophic functions, acting on Schwann cells, and promoting angiogenesis.

引用

页码：183 / 193

页数：11

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