Apoptosis in the small intestinal allograft of the rat

被引:32
作者
Fayyazi, A
Schlemminger, R
Gieseler, R
Peters, JH
Radzun, HJ
机构
[1] UNIV GOTTINGEN,DEPT PATHOL,D-37075 GOTTINGEN,GERMANY
[2] UNIV GOTTINGEN,DEPT IMMUNOL,D-37075 GOTTINGEN,GERMANY
[3] UNIV GOTTINGEN,CLIN GEN SURG,D-37075 GOTTINGEN,GERMANY
关键词
D O I
10.1097/00007890-199704150-00007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background Necrosis and apoptosis are different cell death mechanisms. Necrosis is a pathological process that occurs after destruction of the cell membrane. Apoptosis is a DNA-dependent cell death mechanism, which occurs under physiological and pathological conditions. Although necrosis is a well-defined phenomenon in an acute graft rejection, the occurrence and relevance of apoptosis during this process is largely unknown. Methods. The enterocyte apoptosis rates in allografted (n=24) and isografted (n=24) small intestines of the rat were compared using the in situ end-labeling technique. Results. In situ end-labeling showed a dramatically increased number of apoptotic enterocytes in allografted small intestines, whereas increased labeling could not be observed in isogeneic small intestinal grafts. Conclusions. We suggest that graft rejection-associated apoptosis, in addition to necrosis, plays an important role in the course of organ failure, and that the degree of apoptosis represents another reliable indicator for the diagnosis and prognosis of transplant rejection.
引用
收藏
页码:947 / 951
页数:5
相关论文
共 19 条
[1]  
BUJA LM, 1993, ARCH PATHOL LAB MED, V117, P1208
[2]  
COHEN JJ, 1993, IMMUNOL TODAY, V14, P126, DOI 10.1016/0167-5699(93)90214-6
[3]   BIOCHEMICAL EVENTS IN NATURALLY-OCCURRING FORMS OF CELL-DEATH [J].
FESUS, L .
FEBS LETTERS, 1993, 328 (1-2) :1-5
[4]  
FUKUNAGA RW, 1992, NATURE, V356, P314
[5]   IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].
GAVRIELI, Y ;
SHERMAN, Y ;
BENSASSON, SA .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501
[6]   DETECTION OF DNA FRAGMENTATION IN APOPTOSIS - APPLICATION OF INSITU NICK TRANSLATION TO CELL-CULTURE SYSTEMS AND TISSUE-SECTIONS [J].
GOLD, R ;
SCHMIED, M ;
ROTHE, G ;
ZISCHLER, H ;
BREITSCHOPF, H ;
WEKERLE, H ;
LASSMANN, H .
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1993, 41 (07) :1023-1030
[7]  
ITO H, 1995, TRANSPLANTATION, V60, P794
[8]   APOPTOSIS IN PANCREATIC ALLOGRAFT-REJECTION - ULTRASTRUCTURAL OBSERVATIONS [J].
KNOOP, M ;
MCMAHON, RFT ;
JONES, CJP ;
HUTCHINSON, IV .
EXPERIMENTAL PATHOLOGY, 1991, 41 (04) :219-224
[9]   ACUTE REJECTION OF PORCINE SMALL-BOWEL ALLOGRAFT - AN EXTENDED HISTOLOGICAL SCORING SYSTEM [J].
KUUSANMAKI, P ;
HALTTUNEN, J ;
PAAVONEN, T ;
PAKARINEN, M ;
LUUKKONEN, P ;
HAYRY, P .
TRANSPLANTATION, 1994, 58 (07) :757-763
[10]  
MAJNO G, 1995, AM J PATHOL, V146, P3