Characterization of UT2 cells - The induction of peroxisomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase

被引:32
作者
Engfelt, WH
Shackelford, JE
Aboushadi, N
Jessani, N
Masuda, K
Paton, VG
Keller, GA
Krisans, SK
机构
[1] SAN DIEGO STATE UNIV,DEPT BIOL,SAN DIEGO,CA 92182
[2] GENENTECH INC,DEPT PRECLIN PHARMACOL,S SAN FRANCISCO,CA 94080
关键词
D O I
10.1074/jbc.272.39.24579
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In the liver 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase is present not only in the endoplasmic reticulum but also in the peroxisomes, However, to date no information is available regarding the function of the peroxisomal HMG-CoA reductase in cholesterol/isoprenoid metabolism, and the structure of the peroxisomal HMG-CoA reductase has yet to be determined. We have identified a mammalian cell line that expresses only one HMG-CoA reductase protein and that is localized exclusively to peroxisomes, This cell line was obtained by growing UT2 cells (which lack the en doplasmic reticulum HMG-CoA reductase) in the absence of mevalonate. The cells exhibited a marked increase in a 90-kDa HMG-CoA reductase that was localized exclusively to peroxisomes, The wild type Chinese hamster ovary cells contain two HMG-CoA reductase proteins, the well characterized 97-kDa protein, localized in the endoplasmic reticulum, and a 90-kDa protein localized in peroxisomes. The UT2 cells grown in the absence of mevalonate containing the up-regulated peroxisomal HMG-CoA reductase are designated UT2*. A detailed characterization and analysis of this cell line is presented in this study.
引用
收藏
页码:24579 / 24587
页数:9
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