Molecular detection of GES-2 extended spectrum β-lactamase producing Pseudomonas aeruginosa in Pretoria, South Africa

被引：31

作者：

Weldhagen, GF

Prinsloo, A

机构：

[1] Univ Pretoria, Dept Med Microbiol, Fac Hlth Sci, ZA-0001 Pretoria, South Africa

[2] Natl Hlth Lab Serv, ZA-0001 Pretoria, South Africa

来源：

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS | 2004年 / 24卷 / 01期

关键词：

ESBL; Pseudomonas aeruginosa; PCR detection;

D O I：

10.1016/j.ijantimicag.2003.12.012

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

Screening for and detection of the novel extended spectrum P-lactamase (ESBL), GES-2 produced by Pseudomonas aeruginosa remains a problem in the clinical microbiology laboratory. This study aimed to compare the normally used ESBL screening agent ceftazidime, with molecular detection, to demonstrate the presence of GES-2 ESBL production in clinical isolates of P. aeruginosa. Ceftazidime was found unreliable as an ESBL screening agent, with a specificity of 34.4%, when National Committee for Clinical Laboratory Standards resistance criteria for P aeruginosa were employed. An improved PCR detection method was devised, that amplified a 371 bp segment of bla(GES-2). This should lead to more cost-effective DNA sequencing and sequence interpretation in the laboratory. (C) 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

引用

页码：35 / 38

页数：4

共 16 条

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