Construction and function of two cys146-mutants with high activity, derived from recombinant human soluble B lymphocyte stimulator

被引：5

作者：

Chen, GY ^{[1
]}

Peng, SY ^{[1
]}

Zou, MJ ^{[1
]}

Xu, HL ^{[1
]}

Xu, DG ^{[1
]}

Wang, JX ^{[1
]}

机构：

[1] Beijing Inst Basic Med Sci, Genet Mol Lab, Beijing 100850, Peoples R China

来源：

JOURNAL OF BIOCHEMISTRY | 2004年 / 136卷 / 01期

基金：

中国国家自然科学基金;

关键词：

B cell proliferation; BLyS; cytokine; immunoglobulin secretion; mutation;

D O I：

10.1093/jb/mvh088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

B lymphocyte stimulator (BLyS) is a novel member of tumor necrosis factor (TNF) ligand family that is important in B cell maturation and survival. Previous studies were almost related to the function or mechanism of its wild type. Here, we constructed two site-directed mutants of the recombinant human soluble BLyS, the BY-A and BY-V, and found that BY-V ranked the highest whenever in the process of promoting proliferation of B lymphocytes in vitro or stimulating total serum IgG and IgM secretion in vivo. Besides, assays for the biological responses of human leukemic cell lines to BLyS, BY-A and BY-V demonstrated that they could suppress the proliferation of Raji cells but promote the growth of THP-1. The discovery of BY-V with high activity will help come to a conclusion that the mutation of Cys146 to Val146 might improve the biological activity of BLyS.

引用

页码：73 / 79

页数：7

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