Development of RAPD markers in tritordeum and addition lines of Hordeum chilense in Triticum aestivum

被引：20

作者：

Hernandez, P

Rubio, MJ

Martin, A

机构：

[1] Instituto de Agricultura Sostenible, CSIC

来源：

PLANT BREEDING | 1996年 / 115卷 / 01期

关键词：

Hordeum chilense; Triticum aestivum; XTritordeum; addition lines; molecular markers; RAPD;

D O I：

10.1111/j.1439-0523.1996.tb00870.x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

RAPD markers were developed for octoploid XTritordeum (amphiploid Hordeum chilense x Triticum aestivum) and its parents. Two bread wheats, two H. chilense accessions and the two tritordeums synthesized with them were used. A total of 41 arbitrary decamer primers were tested, yielding 190 products that could be assigned to wheat, 185 to H. chilense and 108 that were nonspecific (present in wheal and barley). A total of 44 products were specific to one H. chilense line and 33 to the other 16 of the former were located on the chromosomes using a set of H. chilense in T. aestivum addition lines. The potential of RAPDs for developing addition lines or the detection of introgressions of H. chilense in bread wheat is discussed.

引用

页码：52 / 56

页数：5

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