Multiplex Ligation-Dependent Probe Amplification of Uveal Melanoma: Correlation with Metastatic Death

被引：131

作者：

Damato, Bertil ^{[1
]}

Dopierala, Justyna ^{[2
]}

Klaasen, Annelies ^{[3
]}

van Dijk, Marcory ^{[4
]}

Sibbring, Julie ^{[5
]}

Coupland, Sarah E. ^{[2
]}

机构：

[1] Royal Liverpool Univ Hosp, Ocular Oncol Serv, Liverpool Ocular Oncol Ctr, St Pauls Eye Clin, Liverpool L7 8XP, Merseyside, England

[2] Univ Liverpool, Dept Pathol, Liverpool L69 3BX, Merseyside, England

[3] Univ Med Ctr Nijmegen, Dept Pathol, Nijmegen, Netherlands

[4] Univ Groningen Hosp, Dept Pathol, Groningen, Netherlands

[5] Liverpool Womens Hosp, Dept Mol Genet, Liverpool, Merseyside, England

来源：

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE | 2009年 / 50卷 / 07期

关键词：

CHOROIDAL MELANOMA; SURVIVAL PROBABILITY; TUMOR PROGRESSION; CHROMOSOME-3; ARM; MONOSOMY-3; HETEROGENEITY; NETWORKS; LOSSES; GAINS;

D O I：

10.1167/iovs.08-3165

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

PURPOSE. To evaluate multiplex ligation-dependent probe amplification (MLPA) of uveal melanoma as a predictive tool for metastatic death. METHODS. Uveal melanoma specimens of 73 patients treated between 1998 and 2000 were included. DNA samples were analyzed with MLPA evaluating 31 loci on chromosomes 1, 3, 6 and 8, and the results were correlated with metastatic death. RESULTS. The patients (27 women; 46 men) had a median age of 60.6 years and a median follow-up of 6.2 years. Metastatic death occurred in 28 patients, correlating most strongly with chromosome 3 losses and gains on 8q (Cox univariate analysis, P < 0.001). Chromosome 6, region p25, gains correlated with good survival (Cox univariate analysis, P = 0.003). Prediction of metastatic death was improved by considering equivocal chromosome 3 losses as abnormal and by taking account of multiple risk factors, such as 8q gains, tumor diameter, and histologic features indicative of high-grade malignancy. CONCLUSIONS. MLPA analysis of uveal melanoma predicts metastatic death if statistically insignificant losses of chromosome 3 are considered together with gains in 8q as well as clinical stage and histologic grade of malignancy. (Invest Ophthalmol Vis Sci. 2009;50:3048-3055) DOI: 10.1167/iovs.08-3165

引用

页码：3048 / 3055

页数：8

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