Ultrastructural morphometry of mammary gland in transgenic and non-transgenic rabbits

被引：7

作者：

Dragin, S.

Pivko, J.

Massanyi, P.

Lukac, N.

Makarevich, A. V.

Paleyanda, R. K.

Chrenek, P. ^{[1
]}

机构：

[1] Slovak Agr Res Author, Nitra, Slovakia

[2] Univ Novi Sad, Fac Agr, Novi Sad, Serbia

[3] Slovak Univ Agr, Fac Food Sci & Biotechnol, Nitra, Slovakia

来源：

ANATOMIA HISTOLOGIA EMBRYOLOGIA | 2006年 / 35卷 / 06期

关键词：

D O I：

10.1111/j.1439-0264.2006.00692.x

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The mammary gland of transgenic animals has been used for the production of recombinant proteins of therapeutic and nutraceutical use. The objective of this study was to compare the ultrastructure of transgenic and non-transgenic rabbit mammary gland tissue. New Zealand White transgenic rabbits were obtained by breeding non-transgenic rabbits with transgenic founder rabbits containing a whey acidic protein-human factor VIII (WAP-hFVIII) transgene integrated into their genome. Samples of mammary gland tissue from lactating rabbit females were isolated by surgical procedures. These samples were examined by optical and electron microscopy and photographs were taken. Measurements of ultrastructural organelles were made from digital images of the mammary cells. No differences were found in the cellular structure of mammary tissue, but significant differences t((0.001)) in the relative volume of mitochondria and vacuoles between transgenic and non-transgenic mammary gland epithelium were observed.

引用

页码：351 / 356

页数：6

共 33 条

[11] Perturbation of β1-integrin mammary gland results in function in involuting premature dedifferentiation of secretory epithelial cells [J].