Biochemistry and molecular genetics of poly-γ-glutamate synthesis

被引：181

作者：

Ashiuchi, M ^{[1
]}

Misono, H ^{[1
]}

机构：

[1] Kochi Univ, Dept Bioresources Sci, Nanko Ku, Kochi 7838502, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2002年 / 59卷 / 01期

关键词：

D O I：

10.1007/s00253-002-0984-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Current research into poly-gamma-glutamate (PGA) and its biosynthesis is reviewed. In PGA-producing Bacillus subtilis, glutamate racemase supplies abundant DL-glutamate, the substrate for PGA synthesis. The pgsBCA genes of PGA-producing B. subtilis, which encode the membrane-associated PGA synthetase complex PgsBCA, were characterized and the enzyme complex was suggested to be an atypical amide ligase based on its structure and function. A novel reaction mechanism of PGA synthesis is proposed.

引用

页码：9 / 14

页数：6

共 46 条

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