IN-VIVO EFFECT OF GROESL ON THE FOLDING OF GLUTAMATE RACEMASE OF ESCHERICHIA-COLI

被引：22

作者：

ASHIUCHI, M

YOSHIMURA, T

KITAMURA, T

KAWATA, Y

NAGAI, J

GORLATOV, S

ESAKI, N

SODA, K

机构：

[1] KYOTO UNIV, INST CHEM RES, UJI, KYOTO 611, JAPAN

[2] TOTTORI UNIV, FAC ENGN, TOTTORI 680, JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1995年 / 117卷 / 03期

关键词：

GLUTAMATE RACEMASE; GROESL; MOLECULAR CHAPERONE;

D O I：

10.1093/oxfordjournals.jbchem.a124735

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The overexpression of the murI (glr) gene, which encodes the glutamate racemase of Escherichia coli, resulted in the formation of inclusion bodies of the enzyme, and little activity was found in the soluble fraction of the transformant cells. The coexpression of the groESL gene with murI caused an in vivo solubilization of glutamate racemase in an active form. We isolated the active enzyme and purified it effectively.

引用

页码：495 / 498

页数：4

共 17 条

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